Abbreviations
Prefacetothesecondedition
Prefacetothefirstedition
SectionA-Cellsandmacromolecules
A1Cellularclassification
A2Subcellularorganelles
A3Macromolecules
A4Largemacromolecularassemblies
SectionB-Proteinstructure
B1Aminoacids
B2Proteinstructureandfunction
B3Proteinanslysis
SectionC-Propertiesofnucleicacids
C1Nucleicacidstructure
C2Chemicalandphysicalpropertiesofnucleicacids
C3Spectroscopicandthermalpropertiesofnucleicacids
C4DNAsupercoiling
SectionD-Prodaryoticandeukaryoticchromosomestructure
D1Prokaryoticchromosomestructure
D2Chromatinstructure
D3Eukaryoticchromosomestructure
D4Genomecomplexity
D5Theflowofgeneticinformation
SectionE-DNAreplication
E1DANreplication:anoverview
E2BacterialDNAreplication
E3Thecellcycle
E4EukaryoticDNAreplication
SectionF-DNAdamage,repairandrecombination
F1Mutagenesis
F2DNAdamage
F3DNArepair
F4Recombination
SectionG-Genemanipulation
G1DNAcloning:anoverview
G2PreparationofplasmidDNA
G3Restrictionenzymesandelectrophoresis
G4Ligation,transformationandanalysisofrecombinants
SectionH-Cloningvectors
H1Designofplasmidvectors
H2Bacteriophagevectors
H3Cosmids,YACsandBACs
H4Eukaryoticvectors
SectionI-Genelibrariesandscrreening
I1Genomiclibraries
I2cDNAlibraries
I3Screeningprocedures
SectionJ-AnalysisandusesofclonedDNA
J1Characterizationofclones
J2Nucleicacidsequencing
J3Polymerasechainreaction
J4Organizationofclonedgenes
J5Mutagenesisofclonedgenes
J6Applicationsofcloning
SectionK-Transcriptioninprokaryotes
K1Basicprinciplesoftranscription
K2EscherichiacoliRNApolymerase
K3TheE.coliσpromoter
K4Transcription,initiation,elongationandtermination
SectionL-Regulationoftranscriptioninprokaryotes
L1Thelacoperon
L2Thetrpoperon
L3Transcriptionalregualationbyalternativeσfactors
SectionM-Transcriptionineukaryotes
M1ThethreeRNApolymerases:characterizationandfunction
M2RNAPolⅠgenes:theribosomalrepeat
M3RNAPolⅢgenes:5SandtRNAtranscription
M4RNAPolⅡgenes:promotersandenhancers
M5GeneraltranscriptionfactorsandRNAPolⅡinitiation
SectionN-Regulationoftranscriptionineukaryotes
N1Eukaryotictranscriptionfactors
N2Examplesoftranscriptionalregulation
SectionO-RNAprocessingandRNPs
O1rRNAprocessingandribosomes
O2tRNAprocessing,RNasePandribozymes
O3mRNAprocessing,hnRNPsandsnRNPs
O4AlternativemRNAprocessing
SectionP-ThegeneticcodeandtRNA
P1Thegeneticcode
P2tRNAstructureandfunction
SectionQ-Proteinsynthesis
Q1Aspectsofproteinsynthesis
Q2Mechanismofproteinsynthesis
Q3Initiationineukaryotes
Q4Translationalcontrolandpost-translationalevenrts
SectionR-Bacteriophagesandeukaryoticviruses
R1Introductiontoviruses
R2Bacteriophages
R3DNAviruses
R4RNAviruses
SectionS-Tumorvirusesandoncogenes
S1Oncogenesfoundintumorviruses
S2Categoriesofoncogenes
S3Tumorsuppressorgenes
S4Apoptosis
Furtherreading
Multiplechoicequestions
Answers
Index