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梁宋平 著 / 湖南师范大学出版社 / 2010-02 / 精装
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上书时间2019-09-09
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梁宋平教授文集:蛋白质与活性多肽探索
《梁宋平教授文集:蛋白质与活性多肽探索》收录了其关于“蛋白质与活性多肽探索”的研究文集,适合从事相关研究工作的人员参考阅读。
梁宋平教授长期从事蛋白质与活性多肽的结构与功能研究以及蛋白质化学结构测定方法学研究。近年来,在蜘蛛毒素和蛋白岳组学研究领域连续获得到6项国家自然科学基金项目和两项"863"项目资助。
第一部分蛋白质化学与蛋白质组学学术论文8-(6-氨基己基)-氨基-5-磷酸腺苷Sepharose4B亲和吸附剂的制备与应用大熊猫乳酸脱氢酶同工酶MA的纯化与某些性质的研究TheprimarystructureofthelactatedehydrogenaseisozymeMafromgiantpandaAcomparisonoftheprimarystructuresoflactatedehydrogenaseisozymesM4fromgiantpanda,redpanda.blackbearanddogCovalentimmobilizationofproteinsandpeptidesforsolid—phasesequencingusingprepackedcapiHarycolumnsSingle.stepelectroelutionofproteinsfromSDS—polyacrylamidegelsandimmobilizationondiisothiocyanate—glassbeadsinprepackedcapillarycolumnsforsolid—phasemicrosequencingAssignmentofthethreedisulfidebridgesofhuwentoxin-I,aneurotoxinfromthespiderSelenocosmiahuwenaChemicalcarboxy-terminalsequenceanalysisofpeptidesusingacetylisothiocyanate世纪之交的蛋白质序列测定技术AssignmentofthethreedisulfidebondsofSelenocosmiahuwenalectinIfromthevenomofspiderSelenocosmiahuwenaIndentificationofvenomproteinsofspiderS.huwenaontwo-dimensionalelectrophoresisgelbyN—terminalmicrosequencingandmassspectrometricpeptidemappingChemicalcarboxyl.terminalsequenceanalysisofpeptidesandproteinsusingtribenzylsilylisothiocyanateAssignmentofthedisulfidebondsofhuwentoxin—IIbyEdmandegradationsequencingandstepwisethiolmodificationC-terminalsequenceanalysisofpeptidesusingtriphenylgermanylisothiocyanateDenolosequencingoftrypticpeptidessulfonatedby4-sulfophenylisothiocyanateforunambiguousproteinidentificationusingpost-sourcedecaymatrix..assistedlaserdesorption/ionizationmassspectrometryProteomecomparativeanalysisofgynogenetichaploidanddiploidembryosofgoldfish(Carassiusauratus)Proteomicanalysisofmouseliverplasmamembrane:useofdifferentialextractiontoenrichhydrophobicmembraneproteinsIntegrationofatwo—phasepartitionmethodintoproteomicsresearchonratliverplasmamembraneproteins第二部分蜘蛛活性多肽研究学术论文Propertiesandaminoacidsequenceofhuwentoxin-I,aneurotoxinpurifiedfromthevenomoftheChinesebirdspiderSelenocosmiahuwena虎纹捕鸟蛛毒的生物学活性鉴定蜘蛛肽类神经毒素研究进展Alectin—likepeptideisolatedfromthevenomoftheChinesebirdspiderSelenocosmiahuwenaProtonnuclearmagneticresonancestudiesonhuwentoxin—IfromthevenomofthespiderSelenocosmiahuwena:2.ThFee.dimensionalstructureinsolutionDEPD:anoveldatabasefordifferentiallyexpressedproteinsSolid’phasesynthesisofhuwentoxin—IanditsstructureandbioactivityanalysisOxidativefoldingofreducedanddenaturedhuwentoxin.IThree—dimensionalstructureofSelenocosmiahuwenalectin—I(SHL.I)fromthevenomofthespiderSelenocosmiahuwenaby2D-NMRPurificationandcharacterizationofhuwentoxin—II,aneurotoxicpeptidefromthevenomoftheChinesebirdspiderSelenocosmiahuwena我国南方捕鸟蛛一新种的生物化学鉴定(蜘蛛目,捕鸟蛛科)Thepresynapticactivityofhuwentoxin—I,aneurotoxinfromthevenomoftheChinesebirdspiderSelenocosmiahuwenaTheeffectofHuwentoxin—IonCa2+channelsindifferentiatedNG108-15cells,apatch—clampstudyFunctionandsolutionstructureofhuwentoxin—IV,apotentneuronaltetrodotoxin(TI'X)一sensitivesodiumchannelantagonistfromChinesebirdspiderSelenocosmihuwenaThestructureofspidertoxinhuwentoxin—11withuniquedisulfidelinkage:evidenceforstructuralevolutioncDNAsequenceanalysisofsevenpeptidetoxinsfromthespiderSelenocosmiahuwena‘Purificationandcharacterizationofraventoxin.Iandraventoxin—III,twoneurotoxicpeptidesfromthevenomofthespiderMacrotheleraveniStructure—activityrelationshipsofhainantoxin—IVandstructuredeterminationofactiveandinactivesodiumchannelblockersAnoverviewofpeptidetoxinsfromthevenomoftheChinesebirdspiderSelenocosmiahuwenaWang[=Ornithoctonushuwena(Wang)]Jingzhaotoxin—III,anovelspidertoxininhibitingactivationofvoltage—gatedsodiumchannelinratcardiacmyocytesAntinociceptiveeffectsofintrathecallyadministeredhuwentoxin—I,aselectiveN—typecalciumchannelblocker.intheformalintestinconsciousratsJingzhaotoxin-I,anovelspiderneurotoxinpreferentiallyinhibitingcardiacsodiumchannelinactivationFunctionandsolutionstructureofHuwentoxin—X,aspecificblockerofN-typecalciumchannels.fromtheChinesebirdspiderOrnithoctonushuwenaSolutionstructureandfunctionalcharacterizationofJingzhaotoxin—XI:AnovelgatingmodifierofbothpotassiumandsodiumchannelsDiscoveryofadistinctsuperfamilyofKunitz—typetoxin(KTI")fromTarantulasProteomeandpeptidomeprofilingofspidervenoms第三部分其他散文与短文寄语即将毕业的同学们从伦敦到诺丁汉——访英随笔之一阿希武德教授——访英随笔之二校园绿地与RAMP——访英随笔之三诺丁汉大学生命科学系——访英随笔之四从数字看诺丁汉大学——访英随笔之五电子邮件(E—Mail)与环球信息网(WWW)——访英随笔之六神经生物学研究室——访英随笔之七揭开生命科学的奥秘心中的铭记——缅怀恩师张龙翔教授开学感赋——与九七级研究生共勉《生命科学研究》发刊词登攀桥赤子亭记《生命科学院开发应用科研成果汇编》前言关于学位论文实验——与新入学生物化学研究生的开学笔谈《生物化学与分子生物学实验教程》前言生命科学对教育科学的启示——2005年在湖南师范大学研究生“麓山论坛”上的讲座蛛海拾贝——在第六届全国生物毒素研讨会上所作学术报告的结束语与2006级生命科学学院研究生共勉致敬爱的谢锦云老师在2008研究室工作会议上的结语一位美国教授的中国情结——记波士顿大学教授,湖南师范大学名誉教授R.A.Lauursen附录后记
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