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正版现货新书 miRNA介导GSPT与Surn蛋白互作及功能调控 9787524500858 肖瑞琳著

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作者: 肖瑞琳著
出版社: 知识产权出版社有限责任公司
ISBN: 9787524500858

出版时间: 2025-07
装帧: 平装
开本: 24cm

作者: 肖瑞琳著
出版社: 知识产权出版社有限责任公司

ISBN: 9787524500858
出版时间: 2025-07

装帧: 平装
开本: 24cm

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作者简介
肖瑞琳，博士，毕业于山西大学生物化学与分子生物学专业，现就职于太原理工大学。一直致力于miRNA功能及其相关分子靶点相互作用的研究，运用基因编辑技术、蛋白质组学分析等生物技术和实验方法，深入探究 miRNA 调控分子靶点的具体机制，进一步揭示 miRNA 调控细胞功能的分子基础。在该领域成果丰硕，已发表相关 SCI 论文十余篇，主持多项省级科研项目，并参与多项国家级科研项目。

目录
第 1 章国内外研究概览································································· 11.1 eRF3/GSPT 蛋白 ······························································· 11.1.1 eRF3 的来源 ···························································· 41.1.2 eRF3 的结构特征 ······················································ 71.1.3 eRF3a 的功能 ·························································121.2 Survivin 蛋白 ··································································241.2.1 Survivin 的来源及其家族 ···········································241.2.2 Survivin 的定位和结构特征 ········································251.2.3 Survivin 的功能 ·······················································281.3 miRNAs ·········································································341.3.1 miRNAs 的来源 ······················································341.3.2 miRNAs 的形成及作用原理 ·······································391.3.3 miRNAs 的功能 ······················································411.4 本书研究目的、意义及创新点 ············································561.4.1 研究目的及意义 ······················································561.4.2 研究创新点 ····························································59第 2 章 miR-144 通过调控 eRF3a/GSPT1 对细胞增殖、迁移的影响研究 ········································································632.1 实验材料 ·······································································652.1.1 实验细胞及菌株 ······················································65miRNA 介导 GSPT 与 Survivin 蛋白互作及功能调控II2.1.2 细胞与菌株的质量控制 ············································ 652.1.3 质粒及 microRNA 模拟物 ········································· 652.1.4 主要的酶及试剂 ····················································· 672.2 实验方法 ······································································· 682.2.1 细胞培养 ······························································ 682.2.2 双荧光素酶报告质粒的构建 ······································ 702.2.3 质粒转染 ······························································ 712.2.4 RNA 干扰 ····························································· 712.2.5 RNA 提取及实时荧光定量 qPCR ································ 722.2.6 miRNA 的提取及实时荧光定量 qPCR ·························· 732.2.7 细胞增殖活性 MTT 检测 ·········································· 742.2.8 划痕实验 ······························································ 762.2.9 Western blot ··························································· 772.3 结果 ············································································· 782.3.1 GSPT1 和 miR-144 在结肠癌细胞 HCT116 细胞中的表达水平检测 ························································ 782.3.2 GSPT1 3′UTR 是 miR-144 的靶物 ······························· 792.3.3 miR-144 通过下调 GSPT1 抑制细胞增殖 ······················ 812.3.4 miR-144 通过下调 GSPT1 抑制细胞迁移 ······················ 832.4 讨论 ············································································· 85第 3 章 Survivin 70 、71 位氨基酸突变体对结肠癌细胞增殖、迁移的分子调控 ······························································· 873.1 材料 ············································································· 893.1.1 实验细胞株、菌株与质粒 ········································· 893.1.2 试剂及工具酶 ························································ 903.2 方法 ············································································· 913.2.1 点突变 ······························································· 913.2.2 pull-down 技术 ······················································· 913.2.3 Survivin 突变体与 eRF3 的共定位 ······························· 923.3 结果 ············································································· 93 目录III3.3.1 Survivin 70、71 位氨基酸突变测序图 ···························933.3.2 Survivin 70、71 位氨基酸的突变体重组蛋白的表达 ·········943.3.3 Survivin 70、71 位氨基酸的突变对与 eRF3a/GSPT1相互作用的影响 ······················································953.3.4 Survivin 70、71 位氨基酸的突变对 Survivin/Survivin二聚体形成的影响 ···················································963.3.5 Survivin 及其 70、71 位氨基酸突变体与 GSPT1 的定位 ·····································································973.3.6 Survivin 70、71 位氨基酸的突变抑制了结肠癌细胞HCT116 增殖 ··························································983.3.7 Survivin 70、71 位氨基酸突变抑制了结肠癌细胞HCT116 迁移 ··························································993.3.8 Survivin 70、71 位氨基酸的突变促进了细胞凋亡 ·········· 1003.3.9 Survivin 70、71 位氨基酸的突变提高了其对顺铂药物的敏感性 ····························································· 1013.4 讨论 ··········································································· 1023.5 结论与展望 ·································································· 106第 4 章人类截短型 GSPT1 蛋白与生存素 Survivin 在体内相互作用及区域分析 ························································ 1114.1 材料 ··········································································· 1124.1.1 菌株与质粒 ·························································· 1124.1.2 寡聚核苷酸 ·························································· 1124.1.3 工具酶及生化试剂 ················································· 1134.1.4 主要试剂的配制 ···················································· 1134.2 方法 ··········································································· 1144.2.1 eRF3 截短体的酵母双杂交重组质粒构建 ···················· 1144.2.2 eRF3 截短体酵母双杂交重组质粒的共转化 ················· 1144.2.3 β-半乳糖苷酶活性分析 ··········································· 1154.2.4 阳性克隆的严谨型筛选 ··········································· 1184.2.5 荧光共定位 ·························································· 120miRNA 介导 GSPT 与 Survivin 蛋白互作及功能调控IV4.3 结果 ············································································ 1234.3.1 eRF3 N 末端区域截短设计 ······································· 1234.3.2 截短体构建方案 ···················································· 1234.3.3 实验设计原理 ······················································· 1254.3.4 截短体功能预测 ···················································· 1254.3.5 截短型 eRF3a/GSPT1、eRF3b/GSPT2 酵母双杂交重组质粒的构建和鉴定 ·············································· 1254.3.6 β-半乳糖苷酶活性分析 ············································ 1294.3.7 eRF3 与 Survivin 共定位 ·········································· 1304.4 讨论 ············································································ 133第 5 章人类肽链释放因子 eRF3 与生存素 Survivin 在体外相互作用及区域分析 ························································· 1365.1 材料 ············································································ 1365.1.1 菌株与质粒 ·························································· 1385.1.2 寡聚核苷酸 ·························································· 1395.1.3 主要酶及生化试剂 ················································· 1395.1.4 主要试剂的配制 ···················································· 1405.2 方法 ·····

内容摘要
本著作聚焦前沿分子机制，深入剖析miRNA如何精准介导GSPT与Survivin蛋白的互作及其功能调控网络。书中系统阐释了二者在细胞生命活动中的信号交互、调控路径及病理生理意义，为肿瘤等疾病机制研究与靶向干预提供新视角。内容汇集最新科研成果，理论扎实、方法详实，兼具学术深度与创新性。结构严谨、逻辑清晰，是生物医学领域科研人员及高校师生的重要参考书。该书有力推动蛋白互作与miRNA调控领域的研究发展，极具研读与收藏价值。

精彩内容
GSPT(G1 to S phase transition)是一种翻译终止因子,它通过结合eRF1识别终止密码子，参与蛋白质翻译终止过程中肽链的释放，对细胞周期有调控作用。Survivin是凋亡抑制蛋白IAP（inhibitors of apoptosis）家族的最小成员，结构独特，功能多样，并在细胞分化、细胞周期G2/M以及细胞凋亡等途径中起调控作用。miRNA是内源性非编码小RNA，参与细胞分化、增殖、凋亡等一系列生理活动。由于miRNA存在的普遍性、功能的多样性，可以作为研究多功能蛋白的一种新型干扰手段
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正版现货新书 miRNA介导GSPT与Surn蛋白互作及功能调控 9787524500858 肖瑞琳著

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